BLOOD FILM FOR MALARIA PARASITES
To detect the presence of malaria parasites in blood.
Stained thick and films are used to diagnose malaria and other blood parasites.
- Place one large drop of well-mixed blood in the center of a clean glass slide.
- Using the corner of a second slide, carefully spread the drop or the blood over an
area of 10-12mm.
- To determine the thickness of the film, place the slide on a piece of newspaper. Small print should just be visible through the blood film.
- Dry the film at room temperature.
- Prepare 1 in 10 dilution of the stock Giemsa.
- Cover the entire smear with the diluted Giemsa stain and stain for at least 10 minutes.
- Wash gently in buffer (7.2) and leave slide top dry in an upright position at room temperature.
- Examine under 100 x objective lens for the presence or absence of malaria (BLOOD) parasites.
v pH of the Buffer 7.2 must be adhered to.
v Blood smear should not be thick.
v Stain should be filtered before use.
v Slides must be picked at random for re-examination as a quality control measure by the in-charge.
BLOOD FILM FOR MALARIA PARASITES (CONTINUATION)
The system entails using a code of between one and four plus signs as follows:
+ = 1 – 10 Parasites per 100 thick film fields.
++ = 11 – 100 Parasites per 100 thick film fields.
+++ = 1 - 10 Parasites per single thick film fields.
++++ = More than 10 Parasites per single thick film field.
All stages of malaria parasites (i.e. Trophozoites, Gametocytes, Pigments and Schizonts) should be reported.
Malaria parasites present (+) or Malaria parasites Not Seen.