HEALTH

Sunday, 10 April 2011

BLOOD FILM FOR MALARIA PARASITES


BLOOD FILM FOR MALARIA PARASITES

OBJECTIVES:

To detect the presence of malaria parasites in blood.

Stained thick and films are used to diagnose malaria and other blood parasites.

PROCEDURE:

  1. Place one large drop of well-mixed blood in the center of a clean glass slide.

  1. Using the corner of a second slide, carefully spread the drop or the blood over an
      area of 10-12mm.

  1. To determine the thickness of the film, place the slide on a piece of newspaper. Small print should just be visible through the blood film.

  1. Dry the film at room temperature.

  1. Prepare 1 in 10 dilution of the stock Giemsa.

  1. Cover the entire smear with the diluted Giemsa stain and stain for at least 10 minutes.

  1. Wash gently in buffer (7.2) and leave slide top dry in an upright position at room temperature.

  1. Examine under 100 x objective lens for the presence or absence of malaria (BLOOD) parasites.

PRECAUTIONS:

v  pH of the Buffer 7.2 must be adhered to.

v  Blood smear should not be thick.

v  Stain should be filtered before use.

v  Slides must be picked at random for re-examination as a quality control measure by the in-charge.





BLOOD FILM FOR MALARIA PARASITES (CONTINUATION)

REPORTING:
The system entails using a code of between one and four plus signs as follows:
+             =   1 – 10                 Parasites per 100 thick film fields.
++           =   11 – 100            Parasites per 100 thick film fields.
+++         =   1   - 10              Parasites per single thick film fields.
++++       =    More than 10   Parasites per single thick film field.

All stages of malaria parasites (i.e. Trophozoites, Gametocytes, Pigments and Schizonts) should be reported.

Malaria parasites present (+) or Malaria parasites Not Seen.

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